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inverted fluorescence microscope  (Nikon)


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    Structured Review

    Nikon inverted fluorescence microscope
    Inverted Fluorescence Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 11764 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ti2+inverted+fluorescence+microscope/ECLIPSE+Ti2/pmc12934279-126-14-17
    Average 99 stars, based on 11764 article reviews
    inverted fluorescence microscope - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Fluorescence:

    Article Title: Seeding biosensor cell line that reproduces the Alzheimer tau fold
    Article Snippet: .. Images were acquired at 405, 488, and 647 nm on a Ti2-inverted fluorescence microscope (Nikon) using a 10× objective. ..


    Article Title: Single-cell transcriptomic profiling of the whole colony of Botrylloides diegensis: Insights into tissue specialization and blastogenesis.
    Article Snippet: .. The cells were visualized under the far-red channel of a Nikon Ti2 Inverted fluorescence microscope (Fig. S1). .. The cells were preserved at -20°C by adding DMSO (10% final concentration). sc-RNA-seq via 10X Genomics & sequencing Frozen cells were thawed on ice and centrifuged at 1500 × g and 4°C.

    Article Title: Seeding biosensor cell line that reproduces the Alzheimer tau fold
    Article Snippet: .. Images were acquired at 405, 488, and 647 nm on a Ti2-inverted fluorescence microscope (Nikon) using a 63× objective. ..

    Article Title: Seeding biosensor cell line that reproduces the Alzheimer tau fold.
    Article Snippet: Immunofluorescence labelling of the overexpressed HA-tau297-391 was done via incubation with a primary anti-HA antibody (HA.11, BioLegend, 16B12) followed by a secondary Alexa647 anti-mouse IgG antibody (Thermo Fisher Scientific, A-21235), both diluted in blocking buffer (1x PBS, 5% FBS) for 1 h at room temperature. .. Images were acquired at 405, 488, and 647 nm on a Ti2 inverted fluorescence microscope (Nikon) using a 63x objective. ..

    Article Title: Seeding biosensor cell line that reproduces the Alzheimer tau fold.
    Article Snippet: The cells were fixed after 48 h with cold methanol for 3 min at room temperature and stained against HA (Merck, 11867423001) and tubulin (DHSB, 12G10), followed by goat anti-rat Alexa488 (Thermo Fisher Scientific, A-11006) and goat anti-mouse Alexa647 (Thermo Fisher Scientific, A-21235) secondary antibodies. .. Images were acquired at 405, 488, and 647 nm on a Ti2 inverted fluorescence microscope (Nikon) using a 10x objective. .. Nuclei were stained with 1 μg/ml Hoechst dye 33342 (Thermo Fisher Scientific, H3570) for 10 min at room temperature.

    Article Title: Single-cell transcriptomic profiling of the whole colony of Botrylloides diegensis : insights into tissue specialization and blastogenesis
    Article Snippet: .. The cells were visualized under the far-red channel of a Nikon Ti2 Inverted fluorescence microscope ( ). ..

    Microscopy:

    Article Title: Seeding biosensor cell line that reproduces the Alzheimer tau fold
    Article Snippet: .. Images were acquired at 405, 488, and 647 nm on a Ti2-inverted fluorescence microscope (Nikon) using a 10× objective. ..


    Article Title: Single-cell transcriptomic profiling of the whole colony of Botrylloides diegensis: Insights into tissue specialization and blastogenesis.
    Article Snippet: .. The cells were visualized under the far-red channel of a Nikon Ti2 Inverted fluorescence microscope (Fig. S1). .. The cells were preserved at -20°C by adding DMSO (10% final concentration). sc-RNA-seq via 10X Genomics & sequencing Frozen cells were thawed on ice and centrifuged at 1500 × g and 4°C.

    Article Title: Seeding biosensor cell line that reproduces the Alzheimer tau fold
    Article Snippet: .. Images were acquired at 405, 488, and 647 nm on a Ti2-inverted fluorescence microscope (Nikon) using a 63× objective. ..

    Article Title: Seeding biosensor cell line that reproduces the Alzheimer tau fold.
    Article Snippet: Immunofluorescence labelling of the overexpressed HA-tau297-391 was done via incubation with a primary anti-HA antibody (HA.11, BioLegend, 16B12) followed by a secondary Alexa647 anti-mouse IgG antibody (Thermo Fisher Scientific, A-21235), both diluted in blocking buffer (1x PBS, 5% FBS) for 1 h at room temperature. .. Images were acquired at 405, 488, and 647 nm on a Ti2 inverted fluorescence microscope (Nikon) using a 63x objective. ..

    Article Title: Seeding biosensor cell line that reproduces the Alzheimer tau fold.
    Article Snippet: The cells were fixed after 48 h with cold methanol for 3 min at room temperature and stained against HA (Merck, 11867423001) and tubulin (DHSB, 12G10), followed by goat anti-rat Alexa488 (Thermo Fisher Scientific, A-11006) and goat anti-mouse Alexa647 (Thermo Fisher Scientific, A-21235) secondary antibodies. .. Images were acquired at 405, 488, and 647 nm on a Ti2 inverted fluorescence microscope (Nikon) using a 10x objective. .. Nuclei were stained with 1 μg/ml Hoechst dye 33342 (Thermo Fisher Scientific, H3570) for 10 min at room temperature.

    Article Title: Single-cell transcriptomic profiling of the whole colony of Botrylloides diegensis : insights into tissue specialization and blastogenesis
    Article Snippet: .. The cells were visualized under the far-red channel of a Nikon Ti2 Inverted fluorescence microscope ( ). ..

    other:

    Article Title: The intracellular bacterium Orientia tsutsugamushi uses the autotransporter ScaC to activate BICD adaptors for dynein-based motility.
    Article Snippet: LC-MS/MS was performed on an Ultimate U3000 HPLC (ThermoFisher Scientific) hyphenated to an Orbitrap QExactive HFX mass spectrometer (ThermoFisher Scientific).



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    Synthesis and characterization of BA-HPCS@CGRP microspheres based on microfluidic fabrication. A. Fourier transform infrared spectroscopy spectra of the HPCS, 3-Carboxyphenylboronic acid (BA), and BA-HPCS. B. The hydrogel precursors appear as a liquid macroscopically before gelation. C. The hydrogels appear milky white after photo-crosslinking. D. The imaging of BA-HPCS@CGRP microspheres based on microfluidic chips: macroscopic and microscopic observations. E. Particle size distribution of BA-HPCS@CGRP microspheres. F and G. Representative scanning electron <t>microscope</t> images of BA-HPCS@CGRP microspheres. H. The pore size distribution of lyophilized BA-HPCS@CGRP microspheres. I. The releasing of CGRP from BA-HPCS@CGRP in PBS and different glucose conditions (100 mg/dL, 400 mg/dL). J. Representative live/dead <t>fluorescence</t> images of L929 cells after co-culture with microspheres (green calcein-AM for live cells, red propidium iodide for dead cells). K. The quantitative analysis of L929 cell viability co-cultured with microspheres. ns, no significance. ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05; ns, no significance. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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    Image Search Results


    Synthesis and characterization of BA-HPCS@CGRP microspheres based on microfluidic fabrication. A. Fourier transform infrared spectroscopy spectra of the HPCS, 3-Carboxyphenylboronic acid (BA), and BA-HPCS. B. The hydrogel precursors appear as a liquid macroscopically before gelation. C. The hydrogels appear milky white after photo-crosslinking. D. The imaging of BA-HPCS@CGRP microspheres based on microfluidic chips: macroscopic and microscopic observations. E. Particle size distribution of BA-HPCS@CGRP microspheres. F and G. Representative scanning electron microscope images of BA-HPCS@CGRP microspheres. H. The pore size distribution of lyophilized BA-HPCS@CGRP microspheres. I. The releasing of CGRP from BA-HPCS@CGRP in PBS and different glucose conditions (100 mg/dL, 400 mg/dL). J. Representative live/dead fluorescence images of L929 cells after co-culture with microspheres (green calcein-AM for live cells, red propidium iodide for dead cells). K. The quantitative analysis of L929 cell viability co-cultured with microspheres. ns, no significance. ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05; ns, no significance. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Materials Today Bio

    Article Title: Sustained-release CGRP microspheres accelerate diabetic wound healing by synergistically promoting neurovascular regeneration through modulation of macrophage and endothelial cell functions

    doi: 10.1016/j.mtbio.2026.103015

    Figure Lengend Snippet: Synthesis and characterization of BA-HPCS@CGRP microspheres based on microfluidic fabrication. A. Fourier transform infrared spectroscopy spectra of the HPCS, 3-Carboxyphenylboronic acid (BA), and BA-HPCS. B. The hydrogel precursors appear as a liquid macroscopically before gelation. C. The hydrogels appear milky white after photo-crosslinking. D. The imaging of BA-HPCS@CGRP microspheres based on microfluidic chips: macroscopic and microscopic observations. E. Particle size distribution of BA-HPCS@CGRP microspheres. F and G. Representative scanning electron microscope images of BA-HPCS@CGRP microspheres. H. The pore size distribution of lyophilized BA-HPCS@CGRP microspheres. I. The releasing of CGRP from BA-HPCS@CGRP in PBS and different glucose conditions (100 mg/dL, 400 mg/dL). J. Representative live/dead fluorescence images of L929 cells after co-culture with microspheres (green calcein-AM for live cells, red propidium iodide for dead cells). K. The quantitative analysis of L929 cell viability co-cultured with microspheres. ns, no significance. ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05; ns, no significance. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: Following the incubation, fluorescence images were captured using a Nikon inverted fluorescence microscope (Nikon, Japan, Modle: Eclipse Ti2-E).

    Techniques: Fourier Transform Infrared Spectroscopy, Spectroscopy, Imaging, Microscopy, Pore Size, Fluorescence, Co-Culture Assay, Cell Culture